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KMID : 0379120100380010057
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Korean Journal of Mycology
2010 Volume.38 No. 1 p.57 ~ p.61
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Purification of Anti-obesity Lipase Inhibitor from the Fruiting Body of Phellinus linteus
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Lee Jong-Kug
Song Jung-Hwa
Lee Jong-Soo
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Abstract
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For development of anti-obesity nutraceuticals from mushrooms, new anti-obesity lipase inhibitor from Phellinus linteus was purified by systematic solvent extraction, TLC and HPLC and characterized. Methanol extract from P. linteus most effectively inhibited(72.5%) porcine pancreatic lipase and ethyl acetate fraction showed the highest inhibitory activity in the systematic solvent extraction. A lipase inhibitor from the ethyl acetate fraction was purified through following steps including 3 times silica gel chromatography and preparative HPLC. The purified lipase inhibitor was a yellowish geen powder and its IC50 value was 175 ng. Its molecular weight by MALDI-TOF-MS was 523.06 Da and showed maximal absorption spectrum at 225.1 nm.
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KEYWORD
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Anti-obesity, Lipase inhibitor, Phellinus linteus, Purification
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